Peptidyl-prolyl isomerase Cyclophilin71 promotes SERRATE phase separation and miRNA processing in Arabidopsis
Abstract
In plants, SERRATE (SE) phase separates to drive dicing body (D-body) assembly and promote miRNA processing. However, how SE phase separation is regulated remains unknown. Here, we identified that Cyclophilin71 (CYP71), a peptidyl-prolyl isomerase (PPIase), positively regulates miRNA processing through a forward genetic screening. Interestingly, CYP71 interacts with SE and enhances its phase separation to promote the formation of D-bodies and increase the activity of the Dicing complex, thereby promoting miRNA processing. The function of CYP71 in miRNA processing is dependent on its PPIase activity. These findings reveal orchestration of miRNA processing by a cyclophilin protein and suggest regulation of SE phase separation by peptidyl-prolyl cis-trans isomerization, a structural mechanism.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 2023
- DOI:
- 10.1073/pnas.2305244120
- Bibcode:
- 2023PNAS..12005244Z