Cross-linker design determines microtubule network organization by opposing motors

Inheritance of genetic material following chromosome duplication in eukaryotic cell division is coordinated by the spindle apparatus. The spindle is a highly interconnected network of microtubule filaments that are cross-linked by different types of molecular motors. How the different motors cooperate to organize the spindle network is not understood. Here, we show that an asymmetric cross-linker design can confer bifunctionality to a mitotic motor in the presence of other motors. The asymmetric motor supports both extensile and contractile microtubule network behaviors as observed in different parts of the spindle. These findings define rules controlling the generation of active microtubule networks and allow us to better understand how motors cooperate to organize the correct spindle architecture when a cell divides.