High Performance Liquid Chromatography-Determination of Different Speciations of Arsenic in Cosmetics by Inductively Coupled Plasma Mass Spectrometry

There is a way to determine four arsenic speciations simultaneously in cosmetic by using liquid chromatography (HPLC) with inductively coupled plasma mass spectrometer (ICP-MS). After being extracted by phosphoric acid solution, the samples were separated by PRP-X100 anion chromatographic column. The mobile phase was aqueous solution (pH=6.0) containing 1% methanol (V/V), 2.0 mmol/L NaH2PO4 and 0.2 mmol/L ethylene diamine tetraacetic acid (EDTA). The octopole reaction cell was adopted to eliminate mass spectrum interferences. The separation of AsIII､DMA､MMA and AsV was achieved within 12 min with flow rate of 1.00 mL/min and sample size of 100 μL. The recovery rates of AsIII, DMA, MMA and As Vranged from 80.6% to 107.2% and the relative standard deviations were less than 6.24% when the spiked amount was 0.10∼ 1.00 μg in different kinds of cosmetic samples. The developed method has the advantage of simplicity, sensitivity and good reproducibility, and can be used for the determination of arsenic speciations simultaneously in cosmetics.