Microwave synthesis of nitrogen and sulfur co-doped carbon dots for the selective detection of Hg2+ and glutathione
Abstract
In this present work, nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared from thiomalic acid as the carbon and sulfur source and NSCDs were used conveniently and sensitively to monitor Hg2+ and glutathione, regarding as a switching mode (turn-on-off) fluorescent sensing probe. The constructed NSCDs were characterized with their morphology and surface functional groups by TEM, XPS and XRD. The average particle size of the as-synthesized NSCDs is 4.4 nm. The fluorescence of NSCDs was efficiently quenched by Hg2+ with excitation and emission wavelength at 380/568 nm, respectively. The sensitive detection method of Hg2+ was established in 0.00-32.0 μM concentration range. The quenched fluorescence of NSCDs@Hg2+ could be recovered after adding GSH. As a result, the concentration of GSH at 0.50-34.0 μM could linearly relate to the change in fluorescence of NSCDs@Hg2+. The Hg2+ and GSH in human urine and serum samples were detected with the recoveries of 98.5%-104.5 and 98.5-103.5%, respectively. Owing to NSCDs favorable biocompatibility and low cytotoxicity, they were successfully applied to detect Hg2+ and GSH in HepG2 cells, indicating that NSCDs have potential applications in biology.
- Publication:
-
Optical Materials
- Pub Date:
- January 2020
- DOI:
- 10.1016/j.optmat.2019.109559
- Bibcode:
- 2020OptMa..9909559L
- Keywords:
-
- Fluorescent probe;
- NSCDs;
- Hg<SUP>2+</SUP>;
- GSH;
- Cell imaging