Dielectric spectroscopy of in-vitro human blood of diabetic and non-diabetic patients through low level laser therapy

The research work investigates the dielectric properties (dielectric constant, dielectric loss and conductivity) of both diabetic and non-diabetic patients’ blood in order to enhance of low level laser therapy (LLLT). Knowing the dielectric properties of blood, profile dose threshold exposure duration can improve LLLT for diabetic mellitus disease. This was achieved, using impedance analyser 4294A, frequency range 40 kHz - 30 MHz. Measurements were taken before and after blood irradiated with a portable diode-pumped solid state laser of wavelength 532 nm at power of 60 mW in standard cuvettes. Control diabetic patient’s bloods were high in dielectric parameter compared with the control non-diabetic patient’s blood. After exposure for 5 and 10 minute’s duration, the value of dielectric loss for the blood exposed for 5 minutes duration appreciated significantly. This is attributed to the energy acquired by haemoglobin and oxygen activation that increased cell membrane resistance, protecting the K⁺ions efflux and thus prevent perilous balance of Ca⁺² ions. Laser power of 60 mW for 5 minutes exposure proved effective in LLL diabetic mellitus therapy.