Hypoxia tolerance in the Norrin-deficient retina and the chronically hypoxic brain studied at single-cell resolution
Abstract
Chronic CNS hypoxia is a characteristic of diverse vascular disorders and environmental conditions. There is strong physiologic evidence that neurons and glia mount a protective response in the face of hypoxic stress. We used single-cell RNA sequencing and metabolic profiling to study the hypoxia responses of neurons and glia in the retina in a mouse model of familial exudative vitreoretinopathy, a developmental disorder in which retinal vascularization is incomplete. These responses were compared with those induced in the mouse cerebral cortex by a 1-week exposure to low atmospheric oxygen. These experiments reveal a distinctive set of genomic and metabolic responses in the hypoxic retina and a related set of genomic responses in the hypoxic cerebral cortex.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- April 2019
- DOI:
- 10.1073/pnas.1821122116
- Bibcode:
- 2019PNAS..116.9103H