Multiplexed profiling of single-cell extracellular vesicles secretion
Abstract
Extracellular vesicles (EVs) are cell-derived nanosized particles medicating cell-cell communication and transferring biology information molecules like nucleic acids to regulate human health and diseases. Conventional methods for EV surface marker profiling can't tell the differences in the quantity and phenotypes of EV secretion between cells. To address this need, we developed a microchip platform for profiling an array of surface markers on EVs from large numbers of single cells, enabling more-comprehensive monitoring of cellular communications. Single-cell EV secretion assay led to previously unobserved cell heterogeneity underlying EV secretion, which might open up avenues for studying cell communication and cell microenvironment in both basic and clinical research.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 2019
- DOI:
- 10.1073/pnas.1814348116
- Bibcode:
- 2019PNAS..116.5979J