Effect of polydiacetylene-based nanosomes on cell viability and endocytosis
Abstract
Polydiacetylene-based nanoparticles have been developed as nanocarriers for various bio-applications. However, how nanocarriers enter the cell environment and affect cell viability has not yet been considerably explored. In this study, polydiacetylene-based nanoliposomes (nanosomes) were electrostatically complexed with rhodamine fluorophores. Based on real-time cell imaging and cell viability assessment, the most highly polymerized nanosomes were found to be less toxic to cells. Moreover, it was revealed that the rhodamine/polydiacetylene nanosome complex dissociates at cell environment, the polydiacetylene nanosome penetrates into cells, as suggested by the fluorescence observed in confocal microscopy images.
- Publication:
-
Nanotechnology
- Pub Date:
- June 2019
- DOI:
- 10.1088/1361-6528/ab0ccf
- Bibcode:
- 2019Nanot..30x5101P
- Keywords:
-
- nanosome;
- electrostatic complex;
- cell viability;
- endocytosis