Fluorescent labeling of bacteriophage T7 by CRISPR-Cas9
Abstract
The objective of this work was to develop a simple method for coupling a fluorescent protein with a bacteriophage capsid. The Escherichia coli bacteriophage T7 was chosen as a model one. The method, based on CRISPR-Cas9 system and the λ-Red recombination, allowed to insert the gene of enhanced yellow fluorescent protein into the phage genome.
- Publication:
-
Journal of Physics Conference Series
- Pub Date:
- November 2019
- DOI:
- 10.1088/1742-6596/1400/3/033005
- Bibcode:
- 2019JPhCS1400c3005Z