A new turn-on fluorescent probe with ultra-large fluorescence enhancement for detection of hydrogen polysulfides based on dual quenching strategy

Based on dual quenching strategy (ESIPT inhibited quenching and PET quenching), we have developed a new turn-on fluorescent probe 1. Combining 3-(benzo[d]thiazol-2-yl)-10-butyl-10H-phenothiazin-2-ol (dye 2) as the fluorophore and 2-fluoro-5-nitro-benzoic as the recognition moiety, probe 1 had feature of notable large Stokes shift, highly sensitivity and selective for monitoring H₂S_n with remarkable fluorescence enhancement (328-fold) response at 534 nm. Probe 1 exhibited excellent performance in the quantitative detection of H₂S_n with a 137 nm Stokes shift and a low detection limit of 26 nM in solution. Finally, probe 1 was successfully utilized to image H₂S_n in living A549 cells and zebrafish.