Over-expression of Bgl1 from Aspergillus niger in Penicillium oxalicum
Abstract
Penicillium oxalicum is the well-known major workhorse for cellulase production in industry. But the low β-glucosidase activity in P. oxalicum cellulase leads to inefficiency in biomass degradation and limits its further application, which is a major bottleneck in the efficient biomass conversion by cellulase. Previous study reported genetic transformations of P. oxalicum with homologous bgl genes. While, heterologously expressed beta-glucosidase by genetically modifying host strain could also be an effective tool in developing production of cellulases. In our study, the bgl1 gene of Aspergillus niger was heterologously overexpressed in Penicillium oxalicum. The resulted strain C3-1 showed 156.6- and 245.2-fold increased production of β-glucosidase relative to the parent strain PT3-1 and the wild-type (WT)strain 114-2, respectively. Besides, the filter paper assay of strain C3-1 increased at a factor of 8-folds compared to WT, resulting in levels of up to 3.2 U/ml. In summary, genetically engineering P. oxalicum signifcantly improves β-glucosidase activity, which will ultimately lead to a potent strategy to substantially boost the hydrolytic efficiency of the cellulase cocktail.
- Publication:
-
International Symposium on the Frontiers of Biotechnology and Bioengineering (FBB 2019)
- Pub Date:
- June 2019
- DOI:
- 10.1063/1.5110821
- Bibcode:
- 2019AIPC.2110b0027W