Characterisation and genomic sequencing of Escherichia coli from the faeces of the Canis lupus familiaris
Abstract
Goal: The goal of this study is to enlarge and provide new strains of E. coli to the current molecular biology database of samples with E. coli strains from dog samples. This study is a part of a larger research project in the Shuyuan Molecular Biology Laboratory at The ISF Academy.
Background: Escherichia coli is a gram-negative gut entero bacterium. It is normally found in the lower intestine of humans and animals, where it thrives in the gut microbiome of its host. Many strains of E. coli derived from the human gut have been sequenced and researched. However, E. coli strains from animals, specifically, dogs, are less researched. Previous works sequencing E. coli from the dog microbiome has been more focused on sequencing E. coli from dogs with urinary tract infections, while E. coli strains originated from healthy dogs are not as well researched. Methods: Three E. coli samples were obtained from a domestic dog. The dog is a 3-year-old Shiba Inu and Japanese Spitz crossbreed. Each sample went through a process of serial dilution and was screened for E. coli using 3M plates. Colonies were then picked and passaged at least ten passages on LB Agar plates. The isolation of individual bacteria will allow for additional genome sequencing work via the Illumina and Nanopore sequencing protocols.- Publication:
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AGU Fall Meeting Abstracts
- Pub Date:
- December 2019
- Bibcode:
- 2019AGUFMED41B1093M
- Keywords:
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- 0805 Elementary and secondary education;
- EDUCATION