The Whole Genome Sequencing of Rice Seeds Surviving 12.5-day Exposure to Space Environment
Abstract
Space radiation was the most important stressor which can induce biological damage and genetic variation in spaceflight. A great deal is known about the kinds of damage produced in the DNA by ultraviolet radiation, to a lesser extent, by ionizing radiation. On the molecular level these effects to the organism are caused mainly by the damage induced by the ionizing radiation to the DNA of the living cell. The SNP, InDel and SV were often found in DNA by radiation after spaceflight. In this study, sequencing research on whole genome of rice seeds surviving 12.5 days exposure to space environment by HiSeqPE150. Taken the genome sequence ofjapanica rice Nipponbare completed the whole genome determination as a control, samtools and breakdancncer and cnvnator were used to study SNP, InDel, SV and CNV of seeds induced by spaceflight and wild-type rice materials. The mapping rate was 82.54%.The analysis showed that the mutagenic effects by space factors evenly distributed and had positive correlation with sizes of each chromosomes. We found 12,775 SNPs, 4,161 InDel, 1,290 SV and 422 CNV. The mutation type occurred in mutant is that SNPs, InDel, SV and CNV, that showed that the space factors changed the single nucleotide of the rice genome was the main factor. This study laid the foundation for exploring the molecular biological mechanism of the real actual spatial environment.
- Publication:
-
42nd COSPAR Scientific Assembly
- Pub Date:
- July 2018
- Bibcode:
- 2018cosp...42E3833Z