The DNA Damage Repair Genes Expression of Arabidopsis thaliana Seeds Surviving 12.5-day Exposure to Space Environment
Abstract
The microgravity and space radiation were the two important stressors which can induce biological damage and genetic variation in spaceflight. It is emphasized that we must pay high attention to the DNA damage repair pathway. But so far, the molecular biological mechanism of DDR induced by radiation and microgravity were still not clear. In this study, the biological effects of spaceflight were investigated in Arabidopsis thaliana from the levels of phenotypes, cell and molecular. In addition, the expression of Ku70, Rad51, XPD and OGG1 genes, as the important biomarkers, were used to assess the effects of spaceflight on the pathway of DDR. The dry seeds of Arabidopsis of wild type and gravity insensitive mutants (pin2 and pgm-1) were brought to space by SJ10 recoverable satellite for 12.5 days. The results shown that the normal growth of Arabidopsis was influenced after spaceflight for a long time. The genes expressions of DDR pathway was promoted varied by microgravity at different development stages in the wild-type and mutants of Arabidopsis after spaceflight. In summary, this study revealed the combined biological effects and mechanisms induced by space radiation and microgravity in the wild-type and mutants of Arabidopsis after spaceflight. It is of great significance to study the biological effects of the real actual spatial environment.
- Publication:
-
42nd COSPAR Scientific Assembly
- Pub Date:
- July 2018
- Bibcode:
- 2018cosp...42E3832Z