Investigation of periodontal tissues in the simulation of the weightlessness factor
Abstract
"Dry" immersion reproduces changes in physiological functions in the human body, as well as weightlessness. Investigations of periodontal tissues were carried out on the 1st day (background), on the 5th day of immersion (output) and on the 7th day after the end of the immersion. All investigations were performed on an empty stomach, before brushing your teeth. Six healthy male volunteers aged 19 to 26 participated in the investigation. Sampling was conducted locally from 8 points using standard sterile tampons. The concentration of immunoglobulins (sIgA, IgA, IgM) and cytokines (IL-6, IL-8, IL-1_, INF_, FNO_, IL-4) in the oral fluid was determined by an enzyme immunoassay using the appropriate reagent kits of ZAO Vector-Best. For statistical processing of the results of the investigations, the scientific and statistical package "Statistica v6.0" was used. There is a tendency for sIgA to grow on the 5th day of immersion, and on the 7th day after the completion of the immersion, a significant increase in 2-fold IgA (p <0.05) compared with the output. It can be assumed that an increase in sIgA is associated with the appearance of parodontopathogenic microflora in the oral cavity during the experiment. Pro-inflammatory cytokines (IL-8, IL-1_, INF_) make the main contribution to the total pool of cytokines, that is, a pro-inflammatory cytokine shift is observed. Concentration of IL-8 at the output is increased in comparison with the input in 3 times (p <0.05). There is a tendency to increase the concentration of INF_ on the 5th day of immersion, and on the 7th day after the completion of the immersion it significantly increases by 2 times (p <0.05) as compared with the output. Thus, the revealed changes in local immunity indicate a decrease in the protective mechanisms of periodontal tissues and the possibility of activation of inflammatory processes.
- Publication:
-
42nd COSPAR Scientific Assembly
- Pub Date:
- July 2018
- Bibcode:
- 2018cosp...42E1539I