QTY code enables design of detergent-free chemokine receptors that retain ligand-binding activities
Abstract
The QTY (glutamine, threonine, and tyrosine) code-designed detergent-free chemokine receptors may be useful in many applications. The QTY variants may be useful not only as reagents in deorphanization studies but also for designing biologics to treat cancer and autoimmune or infectious diseases. The QTY code allows membrane proteins to be systematically designed through simple, specific amino acid substitutions. The QTY code is robust and straightforward: It is the simplest tool to carry out membrane protein design without sophisticated computer algorithms. Thus it can be used broadly. The QTY code has implications for designing additional G protein-coupled receptors and other membrane proteins or, potentially, for rendering water-insoluble and aggregated proteins soluble.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- September 2018
- DOI:
- 10.1073/pnas.1811031115
- Bibcode:
- 2018PNAS..115E8652Z