A new tool for imaging and reconstruction of optically cleared invertebrate animals
Abstract
The invertebrate animal group reaches more than 90% of all living organisms and includes more than 1.3 million described species. In zoological and morphological studies, big size animals and their deep anatomy investigations are limited by examination of serially sectioned material using different microscopy techniques. Whole-mount visualization such huge biological samples are problematic using the classical protocol of immunostaining and microscopy techniques. Here we demonstrate advantages and disadvantages of DMSO-based immune-staining protocols and better hand optical clearing of invertebrates for the study of inner organs and nervous system anatomy, in particular, that could be useful for studying of morphology in several groups of animals.
- Publication:
-
Journal of Physics Conference Series
- Pub Date:
- September 2018
- DOI:
- 10.1088/1742-6596/1092/1/012088
- Bibcode:
- 2018JPhCS1092a2088M