Correlation between Electrical Field Strength and Pulse Width Analysis on Cell Viability
Abstract
Electric field pulses can make living cell membrane electroporated either reversibly or irreversibly. This study determined the effects of electric field pulses on HeLa cancer cell viability. First set of experiment-Pulses electric fields (control, 200V/cm, 400V/cm, 600V/cm, 800V/cm, and 1000V/cm) with fixed pulse width (200µs) were performed on HeLa cells. Second set of experiment - Pulses width of (control,100µs, 500µs,1ms,5ms,10ms) with constant electric field strength of (600V/cm) were performed on HeLa cells. Number of pulses fixed to 1 for both sets of experiments. The quantitative determinations of cell viability measurement were performed by the aid of haemocytometer and trypan blue staining. The data shows that HeLa cell viability decreases as the electric field strength and pulse width. This is because electric pulses alter the cell’s transmembrane potential, causing disruption of the lipid bilayer, and increase the cell membrane permeability. Efficient electroporation outcome only will be gained by fine tuning the appropriate electric field parameter according to the application needed.
- Publication:
-
Journal of Physics Conference Series
- Pub Date:
- June 2018
- DOI:
- 10.1088/1742-6596/1019/1/012012
- Bibcode:
- 2018JPhCS1019a2012G