Real-time cellular and molecular dynamics of bi-metallic self-therapeutic nanoparticle in cancer cells
Abstract
Since last decades various kinds of nanoparticles have been functionalized to improve their biomedical applications. However, the biological effect of un-modified/non-functionalized bi-metallic magnetic nanoparticles remains under investigated. Herein we demonstrate a multifaceted non-functionalized bi-metallic inorganic Gd-SPIO nanoparticle which passes dual high MRI contrast and can kill the cancer cells through several mechanisms. The results of the present study demonstrate that Gd-SPIO nanoparticles have potential to induce cancer cell death by production of reactive oxygen species and apoptotic events. Furthermore, Gd-SPIO nanoparticles also enhance the expression levels of miRNA-199a and miRNA-181a-7p which results in decreased levels of cancer markers such as C-met, TGF-β and hURP. One very interesting finding of this study reveals side scatter-based real-time analysis of nanoparticle uptake in cancer cells using flow cytometry analysis. In conclusion, this study paves a way for future investigation of un-modified inorganic nanoparticles to purport enhanced therapeutic effect in combination with potential anti-tumor drugs/molecules in cancer cells.
- Publication:
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Applied Nanoscience
- Pub Date:
- February 2018
- DOI:
- 10.1007/s13204-018-0654-7
- Bibcode:
- 2018ApNan...8..115V
- Keywords:
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- Gd-SPIO nanoparticles;
- MRI contrast;
- Cancer treatment;
- Real-time analysis