A fast-response two-photon fluorescent probe for imaging endogenous H2O2 in living cells and tissues
Abstract
As a second messenger, hydrogen peroxide plays significant roles in numerous physiological and pathological processes and is related to various diseases including inflammatory disease, diabetes, neurodegenerative disorders, cardiovascular disease and Alzheimer's disease. Two-photon (TP) fluorescent probes reported for the detection of endogenous H2O2 are rare and most have drawbacks such as slow response and low sensitivity. In this report, we demonstrate a simple H2O2-specific TP fluorescent probe (TX-HP) containing a two-photon dye 6-hydroxy-2,3,4,4a-tetrahydro-1H-xanthen-1-one (TX) on the modulation of the ICT process. The probe exhibits a rapid fluorescent response to H2O2 in 9 min with both high sensitivity and selectivity. The probe can detect exogenous H2O2 in living cells. Furthermore, the probe is successfully utilized for imaging H2O2 in liver tissues.
- Publication:
-
Spectrochimica Acta Part A: Molecular Spectroscopy
- Pub Date:
- February 2018
- DOI:
- 10.1016/j.saa.2017.09.034
- Bibcode:
- 2018AcSpA.190..353L
- Keywords:
-
- Two-photon;
- Fluorescent probe;
- Endogenous H<SUB>2</SUB>O<SUB>2</SUB>;
- Living cells and tissues