Expansion microscopy of zebrafish for neuroscience and developmental biology studies
Abstract
We explore the utility of expansion microscopy (ExM) in neuroscience and developmental biology using the zebrafish model. Regarding neuroscience studies, ExM enables the tracing of cellular processes in the zebrafish brain, as well as the imaging of synapses and their biomolecular content and organization. Regarding development, ExM enables the resolving of nuclear compartments, particularly nuclear invaginations and channels, and helps relate such cellular nanostructures to proteins of the cytoskeleton during embryogenesis.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 2017
- DOI:
- 10.1073/pnas.1706281114
- Bibcode:
- 2017PNAS..11410799F