Computer processing of large datasets in the diagnosis of cancer micrometastases in the bone marrow
Abstract
To detect disseminated tumor cells (DTC) in the bone marrow in patients with solid tumors by flow cytometry it is necessary to analyze a very large number of cells (up to 100 million myelokaryocytes). We have proposed the use of a new generation flow cytometers with acoustic focusing, making it possible to analyze up to 20 mln events in one file. To improve the accuracy of our analysis we used fluorochromes with non-overlapping emission spectra, which makes it possible to minimize the need of setting compensation of cytometer. Identification of the target cell population detected by sequential logic gating.
- Publication:
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Journal of Physics Conference Series
- Pub Date:
- January 2017
- DOI:
- 10.1088/1742-6596/798/1/012142
- Bibcode:
- 2017JPhCS.798a2142B