Primer release is the rate-limiting event in lagging-strand synthesis mediated by the T7 replisome
Abstract
Lagging-strand DNA is replicated in multiple segments called Okazaki fragments, whose formation involves a complex molecular cycle mediated by DNA primase, polymerase, and other replisome components. In addition, synthesis of the lagging strand must occur in lockstep with the leading strand. Using the simple replication system of bacteriophage T7, we found that primer release from the DNA primase domain of T7 primase helicase is a critical regulatory event in the initiation of Okazaki fragments and that the T7 single-stranded binding protein, gp2.5, regulates initiation timing.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- May 2016
- DOI:
- 10.1073/pnas.1604894113
- Bibcode:
- 2016PNAS..113.5916H