Electron tomography reveals the fibril structure and lipid interactions in amyloid deposits
Abstract
Although considerable previous efforts have been dedicated to studying the molecular assembly of individual amyloid fibrils, much less is known about their 3D arrangement within a pathological deposit. In this study, we use electron tomography, an extremely powerful method for studying the detailed structure of cellular assemblies or macromolecular complexes, to unravel the superstructure of fibril networks. The structural views provided by our analysis enable a better understanding of the properties and pathogenic features of amyloid fibrils. The fibril network structure is also a crucial determinant of possible applications of such fibrils in the field of biotechnology or material sciences.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- May 2016
- DOI:
- 10.1073/pnas.1523496113
- Bibcode:
- 2016PNAS..113.5604K