Obtaining DNA aptamers to human interleukin-6 for biomagnetic immunoassay nanosensors
Abstract
We used aptamers, which are functional equivalents of antibodies, in order to develop a nanosensor immunoassay system based on magnetic nanoparticles and a SQUID magnetometer. Selection was used to obtain DNA aptamers to interleukin-6; their affinity to the target protein was characterized by surface plasmon resonance. It was shown that the biotinylated aptamer binds to magnetic nanoparticles that were functionalized with streptavidin.
- Publication:
-
Moscow University Physics Bulletin
- Pub Date:
- January 2016
- DOI:
- 10.3103/S002713491601015X
- Bibcode:
- 2016MUPB...71..135S
- Keywords:
-
- magnetic nanoparticles;
- streptavidin-biotin;
- DNA aptamer;
- aptamer primary structure;
- aptamer secondary structure