Selective Rayleigh light scattering determination of trace quercetin with silver nanoparticles
Abstract
Rayleigh light scattering (RLS) is a simple technique with a high potential of sensitive determination of small organic molecules. We have found that ppb amounts of quercetin (Qu) greatly enhance the RLS of the solution of silver nanoparticles (AgNPs) stabilized with cetyltrimethylammonium bromide (CTAB) or sodium n-dodecyl sulfate (SDS). Enhancement of light scattering is observed only in the presence of an excess of AgNO3, which implies that it is a result of nanoparticle growth; another reason for the enhanced scattering is the aggregation of AgNPs by the analyte that was confirmed by dynamic light scattering technique. The conditions were chosen for the determination of Qu in aqueous solution with the detection limits of 0.01 and 0.03μmolL‑1 and linear ranges of 0.1–1.3 and 0.1–2.0μmolL‑1 for SDS- and CTAB-stabilized AgNPs, respectively; the intra-day RSDs did not exceed 7%. Unexpectedly, other bioflavonoids (rutin, dihydroquercetin, and naringenin) did not change the signal of Qu and did not interfere with its determination in 1:1M ratio (0.5μmolL‑1 each). Other compounds (asparagin, uric acid, urea and some inorganic ions) were also tolerated in high amounts.
- Publication:
-
Journal of Luminescence
- Pub Date:
- November 2016
- DOI:
- 10.1016/j.jlumin.2016.07.020
- Bibcode:
- 2016JLum..179..438U
- Keywords:
-
- AgNPs;
- silver nanoparticles;
- AuNPs;
- gold nanoparticles;
- CTAB;
- cetyltrimethylammonium bromide;
- DLS;
- dynamic light scattering;
- HEPES;
- 4-(2-hydroxyethyl)-piperazine-1-ethanesulfonic acid;
- MOPS;
- 3-(N-morpholino)propanesulfonic acid;
- Qu;
- quercetin;
- RLS;
- Rayleigh light scattering;
- Silver nanoparticles;
- Rayleigh light scattering;
- Quercetin determination;
- Bioflavonoid;
- Sodium n-dodecyl sulfate;
- Cetyltrimethylammonium bromide