Capacitive aptamer–antibody based sandwich assay for the detection of VEGF cancer biomarker in serum
Abstract
Selected anti-VEGF aptamer that specifically bind VEGF protein, a cancer biomarker. Fabricated an aptamer based biosensor comprised of gold IDE capacitor arrays as transducers. Developed a unique aptamer–antibody sandwich assay for VEGF protein detection. Aptamer-target complex on sensors was sandwiched by antibody coated magnetic particles. Enhanced signal generated was measured as a change in capacitance/impedance.
- Publication:
-
Sensors and Actuators B: Chemical
- Pub Date:
- March 2015
- DOI:
- 10.1016/j.snb.2014.12.040
- Bibcode:
- 2015SeAcB.209..645Q
- Keywords:
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- VEGF;
- Aptamer;
- Cancer biomarker;
- Biosensor;
- Capacitance