Electrochemical detection of a single cytomegalovirus at an ultramicroelectrode and its antibody anchoring
Abstract
The need for rapid, dependable, and sensitive detection of biological threats is ever increasing. Relatively arduous techniques, with varying degrees of sensitivity, exist for the detection of pathogens, including ELISA, electrogenerated chemiluminescence methods, sensitive PCR techniques, culturing, and microscopy. Here, we extend the observation of particle collisions on ultramicroelectrodes to murine cytomegalovirus. We further present an electrochemical technique for the specific detection of low concentrations of a virus by observing the effect of virus and antibody-specific polystyrene bead binding. This work, in principle, provides a framework for the detection of any biologically relevant antigen.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- April 2015
- DOI:
- 10.1073/pnas.1504294112
- Bibcode:
- 2015PNAS..112.5303D