Facile fabrication of CdSe/CdS quantum dots and their application on the screening of colorectal cancer
Abstract
In this paper, a facile aqueous route to water-soluble CdSe/CdS quantum dots (QDs) under mild conditions has been developed. The samples were characterized by means of transmission electron microscopy, energy-dispersive X-ray spectroscopy, and photoluminescence (PL) spectroscopy. The PL property of the QDs can be controlled by adjusting the reaction time. The CdSe/CdS QDs after 48-h reaction with size of 5 nm have the strongest PL intensity located at 553 nm, and the highest quantum yield of 19.9 %. The obtained QDs were applied for the colorectal cancer screening. The QDs could be conjugated with antibody of aldo-keto reductase family 1, member B10 (AKR1B10) for the detection of AKR1B10. The AKR1B10 in PBS/5 % serum solution with concentration of 1 ng/mL could be well calibrated, and the limit of detection could be lower than 0.05 ng/mL.
- Publication:
-
Journal of Nanoparticle Research
- Pub Date:
- November 2015
- DOI:
- 10.1007/s11051-015-3224-x
- Bibcode:
- 2015JNR....17..430C
- Keywords:
-
- CdSe/CdS quantum dots;
- Water soluble;
- Colorectal cancer;
- Aldo-keto reductase family 1;
- member B10 (AKR1B10);
- Nanomedicine;
- Health effects