PILRα and PILRβ have a siglec fold and provide the basis of binding to sialic acid
Abstract
The paired immunoglobulin-like type 2 receptor α (PILRα) and β (PILRβ) are important surface molecules which, upon ligand binding, can deliver opposing signals to modulate the host immune responses. In this study, we elucidated the molecular basis on the ligand binding of PILRs by systematic structural and functional assays. Both PILRα and PILRβ show a typical siglec-like fold but exhibit variant binding avidities for sialic acid (SA). We further identified key residues responsible for SA binding and elucidated the atomic interaction details via a complex crystal structure. In conclusion, the SA recognition mechanism for the PILR receptors has been, for the first time to our knowledge, systematically investigated and clearly presented.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- June 2014
- DOI:
- 10.1073/pnas.1320716111
- Bibcode:
- 2014PNAS..111.8221L