Optical Coherence Microscopy
Abstract
This chapter presents the practical embodiment of two types of optical coherence microscope (OCM) modality that differ by probing method. The development and creation of a compact OCM device for imaging internal structures of biological tissue at the cellular level is presented. Ultrahigh axial resolution of 3.4 μm and lateral resolution of 3.9 μm within tissue was attained by combining broadband radiations of two spectrally shifted SLDs and implementing the dynamic focus concept, which allows in-depth scanning of a coherence gate and beam waist synchronously. This OCM prototype is portable and easy to operate; creation of a remote optical probe was feasible due to use of polarization maintaining fiber. The chapter also discusses the results of a theoretical investigation of OCM axial and lateral resolution degradation caused by light scattering in biological tissue. We demonstrate the first OCM images of biological objects using examples of plant and human tissue ex vivo.
- Publication:
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Handbook of Coherent-Domain Optical Methods
- Pub Date:
- 2013
- DOI:
- 10.1007/978-1-4614-5176-1_27
- Bibcode:
- 2013hcdo.book.1127G
- Keywords:
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- Physics