Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
Abstract
We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.
- Publication:
-
Scientific Reports
- Pub Date:
- March 2012
- DOI:
- 10.1038/srep00306
- Bibcode:
- 2012NatSR...2E.306V