Recognition of the let-7g miRNA precursor by human Lin28B
Abstract
Mammalian homologs of lin28: Lin28 and Lin28B block the post-transcriptional processing of the let-7 family of miRNAs. We report that in vitro the terminal stem-loop region of the let-7g miRNA precursor (pre-let-7g) required to bind Lin28B is restricted to 24 nucleotides (nt) including the 3‧ GGAG motif. Additionally, full length Lin28B is required for efficient binding to pre-let-7g and the stoichiometry of the complex is 1:1. Molecular dynamics (MD) simulations reveal the interactions of the pre-let-7g stem-loop and the GGAG motif in the stem region to the cold shock domain (CSD) and to the zinc knuckle domain (ZKD) of Lin28B, respectively.
- Publication:
-
FEBS Letters
- Pub Date:
- November 2012
- DOI:
- 10.1016/j.febslet.2012.09.034
- Bibcode:
- 2012FEBSL.586.3986S
- Keywords:
-
- Lin28B;
- Let-7g;
- Pre-miRNA