Fluorescent turn-on Zn 2+ sensing in aqueous and cellular media
Abstract
A naphthalimide-based water soluble Zn 2+ sensor bearing polyamino carboxylate as a metal chelating moiety was synthesized and its application for the selective detection of Zn 2+ ion in 100% aqueous solution is demonstrated. The newly developed fluorescent sensor exhibits selective binding affinity towards Zn 2+ and pH insensitivity in biologically relevant range. Confocal fluorescent microscopy images indicate that this probe works effectively for intracellular Zn 2+ imaging with high cell permeability.
- Publication:
-
Sensors and Actuators B: Chemical
- Pub Date:
- January 2011
- DOI:
- Bibcode:
- 2011SeAcB.160.1489L
- Keywords:
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- Zinc;
- Fluorescent sensor;
- Cell imaging;
- DFT calculations