Proposal of High Density 3D Cell Culture Method Using Micro Structure
Abstract
We have proposed a method for high cell culture throughput by means of a micro 3D scaffold and density with high surface-to-volume ratio, and have successfully demonstrated that the throughput and density of cell culture significantly increased under a finite regime of scaffold size and figuration. We have succeeded in obtaining high density cell culture and also found that the size and figuration of capillaries in the micro 3D scaffolds significantly affect cell culture and surface adhesion.
- Publication:
-
IEEJ Transactions on Electronics, Information and Systems
- Pub Date:
- 2010
- DOI:
- 10.1541/ieejeiss.130.1789
- Bibcode:
- 2010ITEIS.130.1789O
- Keywords:
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- high density 3D cell culture;
- PMMA;
- X-ray lithography