Biophysical Modeling of Fragment Distributions of DNA Plasmids after Heavy Ion Irradiation
Abstract
The investigation of fragment length distributions of plasmid DNA following ion irradiation leads to a better understanding of the induction of DNA damage, particularly the clustering of double strand breaks. We present a model that calculates the fragment distributions of plasmid DNA following heavy ion irradiation by combining the Local Effect Model with a statistical model initially developed for X-rays. The integration of experimental constraints into the model calculations changes the resulting distributions strongly. We find a good agreement of our simulations with experimental fragment distributions based on atomic force microscopy studies. The model provides the means to rapidly predict the results for all ions. It may thus help to find the best ion species in order to demonstrate the impact of the localized energy distribution of particles.
- Publication:
-
Latest Advances in Atomic Cluster Collisions: Structure and Dynamics from the Nuclear to the Biological Scale. Edited by SOLOV'YOV ANDREY ET AL. Published by World Scientific Publishing Co. Pte. Ltd
- Pub Date:
- 2008
- DOI:
- 10.1142/9781848162389_0029
- Bibcode:
- 2008laac.book..389E