Optics far Beyond the Diffraction Limit: Stimulated Emission Depletion Microscopy
Abstract
In this chapter we show that stimulated emission depletion (STED) microscopy and its derivative concepts are able to radically overcome the diffraction barrier in far-field fluorescence imaging, thus disclosing fluorescent details on the macromolecular scale even with diffracted beams of light.
- Publication:
-
Springer Handbook of Lasers and Optics
- Pub Date:
- 2007
- DOI:
- 10.1007/978-0-387-30420-5_16
- Bibcode:
- 2007shlo.book.1091H