Community Genomic Analysis of Strain Variation of a Novel Archaeon in an Acid Mine Drainage Environment
Abstract
Microorganisms play a significant role in acid mine drainage (AMD) generation within the Richmond Mine, Iron Mountain, California. To better understand the contributions of individual microbial species to this process, the assemblies of community genomic data from AMD biofilms were manually curated. Not reported previously is detailed analysis of genomic sequence from G-plasma, an archaeal population from a sample collected from the 5-way location in 2002. The G-plasma population exhibits a small number of differing nucleotide sequences at most genomic locations and comprises multiple genome types. Linkage between these sequence types indicates frequent homologous recombination. As the near complete genome is still in many fragments, the current investigation focused on the 25% of the genome in large, confidently linked pieces. Many predicted proteins from this organism were detected via proteomic analysis. In combination, information about genome heterogeneity and protein expression is providing clues to the role of this population in the biofilm community.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2006
- Bibcode:
- 2006AGUFM.B13C1117Y
- Keywords:
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- 0410 Biodiversity;
- 0418 Bioremediation;
- 0439 Ecosystems;
- structure and dynamics (4815);
- 0448 Geomicrobiology;
- 0456 Life in extreme environments