Fluorescence Intercalibration Experiment: a Multi-laboratory Comparison of Correction Procedures for Fluorescence Analysis of Dissolved Organic Matter
Abstract
Measurement of the fluorescence properties of dissolved organic matter (DOM) provides a window into the biological, chemical and physical processes that affect this significant portion of the global carbon pool. Parameters such as fluorescence intensity, quantum yields, peak bandwidth and peak position provide the basis for interpretation of DOM chemical and environmental variability. Generating reliable parameters from fluorescence data requires both correction for instrument bias and standardized experimental methods. The development, publication and use of correction procedures across different fluorometer platforms has proceeded, however the level of variability among corrected fluorescence data in the general DOM community has not been assessed recently. To that end, an intercalibration study was undertaken to examine the current status of correction procedures with the excitation emission matrix spectroscopy technique (EEMS). Analyses of quinine sulfate standard reference material, Suwannee river fulvic acid, and unconcentrated seawater from the Hudson Canyon were performed by 8 participating laboratories. Statistical analysis of fluorescence variability among laboratories will be discussed, along with implications for future fluorescence analysis of DOM.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2006
- Bibcode:
- 2006AGUFM.B11C1040B
- Keywords:
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- 0428 Carbon cycling (4806);
- 0452 Instruments and techniques;
- 0478 Pollution: urban;
- regional and global (0345;
- 4251);
- 0496 Water quality;
- 4894 Instruments;
- sensors;
- and techniques