Nucleation and growth in one dimension. II. Application to DNA replication kinetics
Abstract
Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems: namely, spatial inhomogeneity, measurement noise, and finite-size effects. After evaluating how each of these affects the measurements of I(t) and v , we give guidelines for the design of experiments. These ideas are then discussed in the context of the DNA-replication experiments.
- Publication:
-
Physical Review E
- Pub Date:
- January 2005
- DOI:
- 10.1103/PhysRevE.71.011909
- arXiv:
- arXiv:cond-mat/0408297
- Bibcode:
- 2005PhRvE..71a1909J
- Keywords:
-
- 87.16.Ac;
- 05.40.-a;
- 02.50.Ey;
- 82.60.Nh;
- Theory and modeling;
- computer simulation;
- Fluctuation phenomena random processes noise and Brownian motion;
- Stochastic processes;
- Thermodynamics of nucleation;
- Condensed Matter - Soft Condensed Matter;
- Condensed Matter - Statistical Mechanics
- E-Print:
- 8 pages, 6 figures