Nanofilter array chip for fast gel-free biomolecule separation
Abstract
We report here a microfabricated nanofilter array chip that can size-fractionate sodium dodecyl sulfate (SDS)-protein complexes and small DNA molecules based on the Ogston sieving mechanism. Nanofilter arrays with a gap size of 40-180 nm were fabricated and characterized. Complete separation of SDS-protein complexes and small DNA molecules were achieved in several minutes with a separation length of 5 mm. The fabrication strategy for the nanofilter array chip allows further increasing of the nanofilter density and decreasing of the nanofilter gap size, leading, in principle, to even faster separation.
- Publication:
-
Applied Physics Letters
- Pub Date:
- December 2005
- DOI:
- 10.1063/1.2149979
- Bibcode:
- 2005ApPhL..87z3902F
- Keywords:
-
- 87.80.-y;
- 87.14.Ee;
- 87.14.Gg;
- 36.20.-r;
- Biological techniques and instrumentation;
- biomedical engineering;
- Proteins;
- DNA RNA;
- Macromolecules and polymer molecules