Imaging of unlabelled bacteria at a sensor surface using aluminium-clad integrated waveguide chips
Abstract
An aluminium-clad leaky waveguide was integrated into a miniaturized chip also containing the required flow geometry and optical coupling elements in a robust device that is relatively simple and inexpensive to fabricate. A leaky waveguiding approach is used to generate a region of very intense interrogating illumination that penetrates ~1 µm into the sample layer before decaying rapidly to zero. This enables the detection of unlabelled, non-fluorescent micron-scale particles and bacteria of similar dimensions, by the capture of scattered light resultant from interactions with the intense leaky guided mode. Such interactions occur only at the waveguide/sample interface and not within the bulk of the sample. This device therefore represents a potentially effective platform for rapid and selective bacterial detection in combination with the appropriate binding procedures.
- Publication:
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Measurement Science and Technology
- Pub Date:
- May 2004
- DOI:
- 10.1088/0957-0233/15/5/024
- Bibcode:
- 2004MeScT..15..948M
- Keywords:
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- bacteria imagingleaky waveguideintegrated opticslight scattering