Surface Electrophoretic Cell using ITO conducting walls
Abstract
The distribution of electric field in and near the surface of the electrophoretic cell determines the motion of DNA in the buffer and along the surface. This is a complicated problem , influenced by buffer ion concentration , electrode configuration and surface and substrate conductivities. To provide a more uniform field distribution near the walls of the electrophoretic cell, we constructed a cell with walls made of conducting surface of ITO (Indium Tin Oxide) coated upon polyester (PET) film (Delta Technologies). The ITO film has a sheet resistance of < 10 Ù. The electric field distribution in the cell was measured with a point voltage probe in cells containing TBE buffer. Steady state field calculations approximating the experimental geometry were made for different arrangements of electrodes. The influence on surface electrophoresis using ë- DNA and Hind III digest DNA in different buffer concentrations will be discussed. Support from NSF- MRSEC is gratefully acknowledged
- Publication:
-
APS March Meeting Abstracts
- Pub Date:
- March 2004
- Bibcode:
- 2004APS..MAR.Z8013R