Synthesis and Structural Study of Retinochrome Analogues
Abstract
Structural studies of retinochrome and rhodopsin are useful for understanding the photoisomerization mechanism of the retinal proteins and chromophore-retinal protein interactions. In this study, some double bond-locked with five-membered ring retinal analogues were synthesized and incorporated into retinochrome as the chromophores to investigated the effect of structural changes in retinochrome. The regeneration of the analogue protein from 11-12 double bond locked chromophores afforded pigments and regeneration processes were competed in 2 hours. Although incubation of 13-14 double bond locked chromophore with apo-retinochrome yielded a retinochrome-like pigment which has absorbance of similar shape and lmax with that of retinochrome, the regeneration of this pigment proceeded slower than others. Photoreaction of 13-14 locked retinochrome converted the all-trans chromophore to the 11-cis isomer in 85 transfered to the 13-cis isomer in about 70 photoisomerization in retinochrome seems to have such a high selectivity.
- Publication:
-
APS March Meeting Abstracts
- Pub Date:
- March 2002
- Bibcode:
- 2002APS..MARM33122Z