Microplate Detection of Microorganisms that Indicate Coastal Water Quality
Abstract
A microplate assay was developed to detect microorganisms that indicate coastal water quality. Species-specific probes based on rDNA sequences were immobilized onto well-plate surfaces. Extracted DNA was amplified using universal biotinylated primers, and amplicons were hybridized to the species-specific probes. The method yielded quick colorimetric results and was used to detect a variety of organisms including Karenia brevis, a toxic dinoflagellate that causes red tide in coastal waters of Florida. The assay holds promise as a quick method to simultaneously test water samples for a variety of toxic organisms.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2002
- Bibcode:
- 2002AGUFMOS21B0215G
- Keywords:
-
- 4840 Microbiology;
- 4855 Plankton;
- 4894 Instruments and techniques