Histamine increases the expression of LOX-1 via H2 receptor in human monocytic THP-1 cells
Abstract
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a member of the scavenger receptor family, and is known to be expressed in monocytes/macrophages. We investigated the effect of histamine on the expression of LOX-1 in cells of the human monocytic leukemia cell line THP-1. Histamine as well as forskolin and dibutyryl cyclic AMP (Bt 2-cAMP) stimulated the THP-1 monocytes to express the LOX-1 gene at the transcription level. This histamine effect on LOX-1 gene expression, via the histamine H2 receptor-mediated cAMP signal transduction pathway, was reduced after differentiation of the cells into macrophages, even though forskolin and Bt 2-cAMP still enhanced the gene expression. The alteration of the responsiveness of LOX-1 expression to histamine was related to suppressed expression of the H2 receptor in THP-1 macrophages. The switch of the predominant class of histamine receptors between H1 and H2 would modulate the effects of histamine on LOX-1 gene expression in monocytes and macrophages, and therefore, would play a certain role in the inflammatory aspects of atherogenesis.
- Publication:
-
FEBS Letters
- Pub Date:
- January 2001
- DOI:
- 10.1016/S0014-5793(01)03073-3
- Bibcode:
- 2001FEBSL.508..345T
- Keywords:
-
- Monocyte/macrophage;
- Histamine receptor;
- Lectin-like oxidized low-density lipoprotein receptor-1;
- Cyclic AMP response element;
- Atherosclerosis;
- CRE;
- cyclic AMP response element;
- CREB;
- CRE binding protein;
- Bt 2-cAMP;
- dibutyryl cyclic AMP;
- FCS;
- fetal calf serum;
- HDC;
- histidine decarboxylase;
- LOX-1;
- lectin-like oxidized low-density lipoprotein receptor-1;
- LDL;
- low-density lipoprotein;
- NBT;
- nitroblue tetrazolium;
- PG;
- prostaglandin;
- TPA;
- tetradecanoylphorbol-13-acetate