Fast forward genetics based on virus-induced gene silencing
Abstract
Gene expression in plants can be suppressed in a sequencespecific manner by infection with virus vectors carrying fragments of host genes. Recent developments have revealed that the mechanism of this gene silencing is based on an RNA-mediated defence against viruses. It has also emerged that a related mechanism is involved in the post-transcriptional silencing that accounts for between line variation in transgene expression and cosuppresion of transgenes and endogenous genes. The technology of virus-induced gene silencing is being refined and adapted as a high throughput procedure for functional genomics in plants.
- Publication:
-
Current Opinion in Plant Biology
- Pub Date:
- 1999
- DOI:
- 10.1016/S1369-5266(99)80022-3
- Bibcode:
- 1999COPB....2..109B
- Keywords:
-
- PDS;
- phytoene desaturase;
- PTGS;
- post-transcriptional gene silencing;
- PVX;
- potato virus X;
- RMD;
- RNA-mediated defense;
- TBRV;
- tomato blackring virus;
- TMV;
- tobacco mosaic virus;
- VIGS;
- virus-induced gene silencing