A Single Receptor Encoded by vzg-1/lpA1/edg-2 Couples to G Proteins and Mediates Multiple Cellular Responses to Lysophosphatidic Acid
Abstract
Extracellular lysophosphatidic acid (LPA) produces diverse cellular responses in many cell types. Recent reports of several molecularly distinct G protein-coupled receptors have raised the possibility that the responses to LPA stimulation could be mediated by the combination of several uni-functional receptors. To address this issue, we analyzed one receptor encoded by ventricular zone gene-1 (vzg-1) (also referred to as lpA1/edg-2) by using heterologous expression in a neuronal and nonneuronal cell line. VZG-1 expression was necessary and sufficient in mediating multiple effects of LPA: [3H]-LPA binding, G protein activation, stress fiber formation, neurite retraction, serum response element activation, and increased DNA synthesis. These results demonstrate that a single receptor, encoded by vzg-1, can activate multiple LPA-dependent responses in cells from distinct tissue lineages.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- May 1998
- DOI:
- 10.1073/pnas.95.11.6151
- Bibcode:
- 1998PNAS...95.6151F