Absorption and emission spectroscopy in the analysis of laser radiation and fast neutron action on chromatin
Abstract
The actions of a 248 mm excimer laser radiation (0.5 - 3 MJ/m2) and of fast neutrons (d(13 MeV) + Be thick target, 5 - 100 Gy) on chromatin (the complex of DNA and proteins of eukaryotic cells) were analyzed. The parameters estimated were: the thermal transition, the fluorescence of chromatin-ethidium bromide complexes, the chromatin intrinsic fluorescence and the energy transfer efficiency between specific fluorescent ligands coupled at chromatin. The mechanism of UV excimer laser action is similar to the fast neutrons action on chromatin. The complex action includes the production of chromatin DNA strand breaks, the protein structure lesion and the chromatin conformation modification.
- Publication:
-
ROMOPTO 1994: 4th Conference in Optics
- Pub Date:
- March 1995
- DOI:
- 10.1117/12.203584
- Bibcode:
- 1995SPIE.2461..405R