Thymic Selection of Cytotoxic T Cells Independent of CD8α-Lck Association
Abstract
The CD8α cytoplasmic domain associates with p56lck, a nonreceptor protein-tyrosine kinase. The biological relevance of CD8α-Lck association in T cell development was tested with transgenic mice generated to express a CD8α molecule with two amino acid substitutions in its cytoplasmic domain, which abolishes the association of CD8α with Lck. The CD8α mutant was analyzed in a CD8-/- background and in the context of the transgenic 2C T cell receptor. The development and function of CD8^+ T cells in these mice were apparently normal. Thus, CD8α-Lck association is not necessary for positive selection, negative selection, or CD8-dependent cytotoxic function.
- Publication:
-
Science
- Pub Date:
- September 1993
- DOI:
- 10.1126/science.8372352
- Bibcode:
- 1993Sci...261.1581C